Information
Catalog number
5522
Full name
Amplite Colorimetric Biotin Quantitation Kit
Size
200 Tests
Price
345.00 €
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Description
Amplite Colorimetric Biotin Quantitation Kit is used for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
Shipping
Room Temperature
Storage
Freezer
Shelflife
6 months
Kit Components
Component A: avidin ; Component B: HABA Assay Buffer; Component C: D-Biotin
Ex (nm)
500 nm
Em (nm)
NA
Personal Protection
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Uses
Laboratory chemicals *For Research Use Only*
Ordering
To order Amplite Colorimetric Biotin Quantitation Kit, please use the Cat. Nr.5522 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Colorimetric Biotin Quantitation Kit
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Conjugation
Biotinylated