Amplite Colorimetric Biotin Quantitation Kit
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Amplite Colorimetric Biotin Quantitation Kit is used for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
Component A: avidin ; Component B: HABA Assay Buffer; Component C: D-Biotin
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Laboratory chemicals *For Research Use Only*
To order Amplite Colorimetric Biotin Quantitation Kit, please use the Cat. Nr.5522 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Colorimetric Biotin Quantitation Kit
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Colorimetric assays or detection use UV absorption or enzymatic color reaction.