Cell Navigator Lysosome Staining Kit *Blue Fluorescence*
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Ambient (Room) temperature
Cell Navigator Lysosome Staining Kit *Blue Fluorescence* is a ready to use kit which is designed to save considerably more assay time when compared to the alternative indoor methods. The easy to follow and user friendly protocol will guide you through each step.
The liquid components of the Cell Navigator Lysosome Staining Kit *Blue Fluorescence* may get entrapped on the walls and seal of the tubes. Before starting the assay briefly spin the vials on a centrifuge to ensure that all of the content is on the bottom. Avoid cycles of freezing and thawing as they may denaturate protein components thus reducing the kits performance. Some of the components of the Cell Navigator Lysosome Staining Kit *Blue Fluorescence* may be photosensitive, therefore exposing them to bright light should be avoided.
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
For cells, cell lines and tissues in culture till half confluency.
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.