Information

Catalog number

NE001-Bio

Full name

Rabbit anti Saccharomyces cerevisiae S-Acetyl Coenzyme A synthetase, conjugated with Biotin, Primary Antibodies

Size

1ml

Price

487.00 €

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Details

Host/Source

Rabbit

Clone Name

Polyclonal

Product Category

Enzymes, Microbiology

Species Reactivity

Saccharomyces cerevisiae

Application

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Immunogen

S-Acetyl coenzyme A synthetase isolated and purified from bakerÍs yeast. FreundÍs complete adjuvant is used in the first step of the immunization procedure.

Purification Method

NA

Shipping Conditions

NA

Storage conditions

Rabbit anti Saccharomyces cerevisiae S-Acetyl Coenzyme A synthetase, conjugated with Biotin, Primary Antibodies philised conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days.

Datasheet link

NA

Description

N terminal acetylation or CH3CO as epigenetic regulation of Rabbit anti Saccharomyces cerevisiae S- Coenzyme A synthetase, conjugated with Primary Antibodies by NATs.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

About

Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus