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Rabbit anti ADAM 10 (CT) (kuz) (TNFa converting enzyme)

Information

Catalog number

X1106P

Full name

Rabbit anti ADAM 10 (CT) (kuz) (TNFa converting enzyme)

Size

100 μg

Price

369.00 €

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Details

Category

Primary Antibodies

Long description

Proinflammatory cytokine tumor necrosis factor- (TNF- ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF- , notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF- , notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Antibody come from

Synthetic peptide corresponding to amino acids 732 to 748 off the human ADAM10. Immungen sequence is identical in bovine and rat and differs from the mouse protein by one amino acid.

Other description

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification

Clone

Polyclonal

Antigen-antibody binding interaction

Rabbit anti ADAM 10 (CT) (kuz) (TNFa converting enzyme) Antibody

Antibody is raised in

Rabbit

Antibody's reacts with

Human, Mouse, Rat

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Western Blot

Antibody's suited for

Detects ADAM10 by Western blot at 0.25 to 1 µg/ml. Detects an 85 kDa band can be detected in Jurkat cell lysates which may represent precursor protein. A faint 60 kDa band is also detected in some cell lines, including Jurkat, which may be processed mature protein. Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Rosendahl, M.S., et al., Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J. Biol. Chem. 1997, 272, 24588-24593_x000B__x000B_2. Pan, D. & Rubin, G.M., Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997, 90, 271-280_x000B__x000B_3. Qi, H., et al., Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999, 283, 91-94 _x000B__x000B_4. Howard, L., et al., Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem. J. 1996, 317, 45-50

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

About

Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus