Information
Catalog number
X1661E
Full name
MEG-1 (637-926)/PTPN4 N-Terminal GST Tag - Active Enzyme
Size
20 μg
Price
426.00 €
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Category
Proteins & Peptides
Long description
MEG-1, also know as Serine/threonine phosphatase 4 regulatory subunit 1, PPP4R1 or PP4R1 is crucial to cellular function. The catalytic subunit of MEG-1 or protein phosphatase 4 (PP4C) is 65% identical to PP2AC at the amino acid level and has been placed in the type 2A family of phosphatases. MEG-1 has been highly conserved between species sharing 91% amino acid identity between human and Drosophila. MEG-1 is predominantly localized in the nucleus in rat brain and liver but is most highly expressed in testis. MEG-1 is an essential enzyme in the development of Drosophila embryos.
Antibody come from
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Ser133 of CREB
Other description
Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol .
Clone
not specified
Antigen-antibody binding interaction
MEG-1 (637-926)/PTPN4 N-Terminal GST Tag - Active Enzyme
Antibody is raised in
E.coli
Antibody's reacts with
Human
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody's specificity
No Data Available
Application
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Antibody's suited for
Antibody can be used for Western blotting and dot blotting (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions.
Storage
-20ºC
Relevant references
1. Abel, T. and Kandel, E. 'Positive and negative regulatory mechanisms that mediate long-term memory storage,' Brain Res. Rev. 26, 360-378 (1998)._x000B__x000B_2. Hunter, T. and Karin, 'The regulation of transcription by phosphorylation,' Cell 70, 375-387 (1992)._x000B__x000B_3, Johannessen, M, Delghandi, M.P. and Moens, U. 'What turns CREB on?' Cellular Signaling 16, 1211-1227 (2004)._x000B__x000B_4. Montminy, M. 'Transcriptional regulation by cyclic AMP,' Annu. Rev. Biochem. 66, 807-822 (1997)._x000B__x000B_5. Shaywitz, A.J. and Greenberg, M.E. 'CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals,' Annu. Rev. Biochem. 68, 821-861 (1999)._x000B__x000B_6. Silva, A.J., Kogan, J.H., Frankland, P.W. and Kida, S. 'CREB and memory,' Annu. Rev. Neurosci. 21, 127-148 (1998)._x000B__x000B_7. Soderling, T.R. and Derkach, V.A. 'Postsynaptic protein phosphorylation and LTP,' Trends Neurosci. 23, 75-80 (2000)._x000B__x000B_8. Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: Signal transmission from the cell surface_x000B_to the nucleus. Curr Biol 5:747-757. _x000B__x000B_9. Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME_x000B_(2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events._x000B_Neuron 34:221-233.
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.