Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme


Catalog number


Full name

Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme


10 μg


426.00 €

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Proteins & Peptides

Long description

Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

Antibody come from

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytoskeletal preparation of OTN-11 ovarian carcinoma cells and SP2/0-Ag14 mouse myeloma cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.


not specified

Antigen-antibody binding interaction

Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme

Antibody is raised in

Sf9 cells

Antibody's reacts with


Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available


Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Antibody's suited for

Antibody can be used for immunohistochemistry on frozen or paraffin-embedded tissue, immunocytochemistry, immunoblotting (1:100-1:1000) and flow cytometry (1:100-1:200). Frozen sections require fixation in methanol. Paraffin-embedded sections require pretreatment with 0.1% pronase/0.1% trypsin in PBS or 0.5% pepsin in 0.01N HCl. Optimal concentration should be evaluated by serial dilutions.



Relevant references

1. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55._x000B__x000B_2. van Niekerk, C. C., et al. (1991). Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues, J Pathol 165, 145-52._x000B__x000B_3. van Niekerk, C. C., et al. (1991). Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455- 63._x000B__x000B_4. van de Molengraft, F. J., et al. (1993). OV-TL 12/30 (keratin 7 antibody) is a marker of_x000B_glandular differentiation in lung cancer, Histopathology 22, 35-8._x000B__x000B_5. van Niekerk, C. C., et al. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies, Cancer Detect Prev 21, 247-57._x000B__x000B_6. Torenbeek, R., et al. (1998). Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma, Histopathology 32, 20-7.

Protein number

see ncbi


This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.


Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.