Information
Catalog number
X2076E
Full name
Ceramide Kinase (CERK) - Active Enzyme
Size
10 μg
Price
426.00 €
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Category
Proteins & Peptides
Long description
Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.
Antibody come from
Synthetic protein made to the 3rd cytoplasmic domain of human Muscarinic Acetylcholine Receptor M3 linked to KLH
Other description
Phosphate buffered saline containing 0.1% sodium azide.
Clone
not specified
Antigen-antibody binding interaction
Ceramide Kinase (CERK) - Active Enzyme
Antibody is raised in
Sf9 cells
Antibody's reacts with
Human
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody's specificity
No Data Available
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Antibody's suited for
Antibody can be used for immunohistochemistry (5-10µg/ml). Optimal concentration should be evaluated by serial dilutions.
Storage
-20ºC
Relevant references
no information yet
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.