Monoclonal IgM Purification - 50 ml ascites/1000 ml TC Supernatant
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Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. virusys supplies purification kits and ultra pure reagents.