Information

Catalog number

K170-50

Full name

Apoptotic DNA Ladder Isolation Kit

Size

50 assays

Price

449.00 €

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Details

Kit's description

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apoptotic DNA Ladder Extraction Kit provides an easy and sensitive means for detecting DNA fragmentation in apoptotic cells. The new procedure selectively extract DNA ladders without interference of intact genomic DNA, which significantly increase the cell numbers that can be can be extracted and load on one agarose gel well, therefore increase the detection sensitivity.

Highlights

• Detection method: Agarose gel electrophoresis (with Ethidium Bromide) • Species reactivity: Mammalian • Kit size: 50 assays • Applications: Extraction of apoptotic DNA ladders without interference of intact genomic DNA

Kit's benefits

• Simple one-step procedure; takes only 2-4 hours • Fast and convenient • The kit can extract apoptotic DNA ladder from both tissues and cells with as little as 5% or less apoptotic cell population.

Category

Apoptosis Assay Kit

Kit's other name

none

Contents

• DNA Ladder Extraction Buffer • Enzyme A Solution • Enzyme B (Lyophilized) • Ammonium Acetate Solution • DNA Suspension Buffer

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Samples tested

Mammalian cell types and tissues undergoing apoptosis