Information

Catalog number

K188-25

Full name

CaspGLOWTM Fluorescein Active Caspase-8 Staining Kit

Size

25 assays

Price

237.00 €

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Details

Kit's description

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit provides a convenient means for sensitive detection of activated caspase-8 in living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.

Highlights

• Detection method: Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Sensitive detection of activated caspase:8 in living cells.

Kit's benefits

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The FITC label allows for direct detection of the activated caspases in apoptotic cells

Category

Apoptosis Assay Kit

Kit's other name

none

Contents

• FITC-IETD-FMK • Wash Buffer • Z-VAD-FMK

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

6-12 months

Species reactivity

see datasheet

Applications

Sensitive detection of activated caspase:8 in living cells.

Samples tested

Live cells

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.