Information

Catalog number

K190-25

Full name

CaspGLOWTM Red Active Caspase Staining Kit

Size

25 assays

Price

237.00 €

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Details

Kit's description

Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.

Highlights

• Detection method: Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Detection of activated caspases in living cells.

Kit's benefits

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The red fluorescence label allows for direct detection of activated caspases in apoptotic cells

Category

Apoptosis Assay Kit

Kit's other name

none

Contents

• Red-VAD-FMK • Wash Buffer • Z-VAD-FMK

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

6-12 months

Species reactivity

see datasheet

Applications

Detection of activated caspases in living cells.

Samples tested

Live cells

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Conjugation

red dye