Information
Catalog number
K199-25
Full name
CaspGLOWTM Red Active Caspase-9 Staining Kit
Size
25 assays
Price
237.00 €
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Kit's description
Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase-9 Staining Kit provides a convenient means for detecting activated caspase-9 in living cells. The assay utilizes a caspase-9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.
Highlights
• Detection method: Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Detection of activated caspase:9 in living cells.
Kit's benefits
• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The fluorescence label allows detection of activated caspase-9 in apoptotic cells
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• Red-LEHD-FMK • Wash Buffer • Z-VAD-FMK
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
6-12 months
Species reactivity
see datasheet
Applications
Detection of activated caspase:9 in living cells.
Samples tested
Live cells
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Conjugation
red dye