Information
Catalog number
K228-100
Full name
Angiotensin I Converting Enzyme (ACE1) Inhibitor Screening Kit
Size
100 assays
Price
864.00 €
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Kit's description
Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. In addition, elevated levels of ACE1 are found in sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher disease, pneumoconiosis, histoplasmosis and miliary tuberculosis. BioVision’s ACE1 Inhibitor Screening Kit can be used to screen for potent inhibitors of ACE1 activity to regulate hypertension. It utilizes the ability of an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide substrate to release a free fluorophore). The released Abz can be easily quantified using a fluorescence microplate reader. In the presence of an ACE1 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE1 inhibitors in a high-throughput format
Highlights
• Detection method : Fluorescence (Ex/Em = 330/430 nm) • Applications: Screening/studying/characterizing inhibitors of ACE1
Kit's benefits
• Simple one-step reaction • Takes only 1-2 hrs • Non-radiometric fluorescent screening process • HTP adaptable
Category
Protease Activity
Kit's other name
none
Contents
• ACE1 Assay Buffer • ACE1 Dilution Buffer • ACE1 Enzyme • ACE1 Substrate • ACE1 Inhibitor (10 mM Captopril)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
not specified
Applications
Screening/studying/characterizing inhibitors of ACE1
Samples tested
Small Molecules/Ligands
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.