Information
Catalog number
K235-50
Full name
Lysosome Purification Kit
Size
50 Extractions
Price
515.00 €
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Kit's description
Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm. BioVision’s Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation
Highlights
Applications: • Isolation of high purity lysosomes from tissues and cultured cells • Lysosome studies like enzyme activity or uptake studies • Protein profiling of lysosomes by SDS:PAGE and Western blot
Kit's benefits
• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation
Category
Cell Fractionation, Lysosome Isolation
Kit's other name
none
Contents
• Lysosome Isolation Buffer • Lysosome Enrichment Buffer • Lysosome Gradient • Protease Inhibitor Cocktail
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Isolation of intact functional lysosomes from tissues and cultured cells • Lysosome studies like enzyme activity or uptake studies • Protein profiling of Lysosomes by SDS-PAGE and Western blot
Samples tested
• Mammalian glands and soft tissues such as liver, kidney, etc. • Cultured eukaryotic cell lines
Tissue
lysosome
Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.