Information

Catalog number

K234-100

Full name

EZExtract™ Core Histone Isolation Kit

Size

100 extractions

Price

277.00 €

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Details

Kit's description

Histones are small alkaline proteins that interact with eukaryotic DNA to form the fundamental subunit of chromatin known as the nucleosome. The core histones H2A, H2B, H3 and H4 form the central spooling unit of the nucleosome; with two copies of each comprising a histone octamer that interacts with the negatively charged DNA. They enable an ordered compaction of the DNA, as well as spatiotemporal control of gene expression and genomic replication throughout mitosis. Post-translational modification of this core complex results in structural changes within the chromatin and epigenetic control of gene expression. BioVision’s EZExtract™ Core Histone Isolation Kit offers an optimized suite of reagents to rapidly extract the total core histones while preserving acetylation from tissues and cultured cells for biochemical and epigenetic analyses. One hundred standard extractions at a scale of 107 cells or 100 mg tissues can be performed with the reagents in this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue

Highlights

Applications: • Convenient and fast isolation of histones from mammalian tissues • Protein profiling, post:translational modification and epigenetic analyses • Protein profiling of histones by SDS:PAGE and Western blot

Kit's benefits

• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate histones with minimal sample preparation

Category

Cell Fractionation, Histone isolation

Kit's other name

none

Contents

• Lysis Buffer • Extraction Reagent • Neutralizing Buffer • DTT (1 M)

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

• Isolation of intact functional core histones from tissues and cultured cells • Protein profiling, post-translational modification and epigenetic analyses • Protein profiling of Histones by SDS-PAGE and Western blot

Samples tested

• Mammalian glands and soft tissues such as liver, spleen, lungs etc. • Cultured eukaryotic cell lines