Information

Catalog number

K258-30

Full name

Apoptotic Cell Isolation Kit

Size

30 isolations

Price

449.00 €

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Details

Kit's description

The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca²ᶧ-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while nonapoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.

Highlights

• Detection method: Magnetic bead bound apoptotic cells can be separated from normal cells • Species reactivity: Mammalian • Applications: Kit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads (MagBeads). The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.

Kit's benefits

• Simple procedure; takes only ~1 hours • Fast and convenient

Category

Apoptosis Assay Kit

Kit's other name

none

Contents

• Annexin V-Biotin • 1X Binding Buffer • Streptavidin-MagBeads • Apoptotic Cell Releasing Buffer

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Kit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads (MagBeads). The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.

Samples tested

Cell cultures (adherent and suspension)

Tissue

cell

Description

For cells, cell lines and tissues in culture till half confluency.