Information

Catalog number

K259-50

Full name

Yeast Mitochondria Isolation Kit

Size

50 assays

Price

399.00 €

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Details

Kit's description

Mitochondria are the power house of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. BioVision’s Yeast Mitochondria Isolation kit will enable fast and easy purification of mitochondria from yeast cells, utilizing yeast cell wall lysis and homogenization. This Mitochondria Isolation Kit is tested for Pichia pastoris & Saccharomyces cerevisiae & can be used to isolate mitochondria from other yeast. Under fermentable media, the yield is ~150-200 µg of mitochondria & under non-fermentable media (e.g. Glycerol) ~200-250 µg of mitochondria from a culture of OD ~20.

Highlights

Application • Isolation of highly pure intact mitochondria from yeast cells. • Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. • Western blot and ELISA

Kit's benefits

• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria

Category

Mitochondria

Kit's other name

none

Contents

• Buffer A • Buffer B • 1 M DTT • Homogenization Buffer • Lysis Enzyme Mix • Storage Buffer • Protease Inhibitor Cocktail

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Inquire

Samples tested

Yeast cells culture.