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Cell Surface Protein Isolation Kit

Information

Catalog number

K295-10

Full name

Cell Surface Protein Isolation Kit

Size

10 reactions

Price

469.00 €

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Details

Kit's description

Cell surface proteins play a major role in signal transduction, cell adhesion, transport and serve as diagnostic and pharmacological targets. BioVision’s Cell Surface Protein Isolation Kit provides a simple and efficient method for the isolation of cell surface proteins. In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using Streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. Biotinylation reagent is cell-membrane-impermeable with an extended spacer arm to reduce steric hindrances associated with streptavidin binding. This convenient kit provides all the required components for optimal labeling and isolation of the cell surface proteins.

Highlights

• Detection method: Western Blotting • Applications: Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies

Kit's benefits

• Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins

Category

Protein Extraction & Purification, cell surface protein isolation

Kit's other name

none

Contents

• Quenching Solution • Lysis Buffer • Wash Buffer • Streptavidin Beads • PBS Tablet • TBS Tablet • Sulfo-NHS-SS-Biotin • DTT

Storage condition

4°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies

Samples tested

Adherent and suspension cells

Tissue

cell

Description

For cells, cell lines and tissues in culture till half confluency.