Information
Catalog number
K295-10
Full name
Cell Surface Protein Isolation Kit
Size
10 reactions
Price
469.00 €
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Kit's description
Cell surface proteins play a major role in signal transduction, cell adhesion, transport and serve as diagnostic and pharmacological targets. BioVision’s Cell Surface Protein Isolation Kit provides a simple and efficient method for the isolation of cell surface proteins. In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using Streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. Biotinylation reagent is cell-membrane-impermeable with an extended spacer arm to reduce steric hindrances associated with streptavidin binding. This convenient kit provides all the required components for optimal labeling and isolation of the cell surface proteins.
Highlights
• Detection method: Western Blotting • Applications: Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies
Kit's benefits
• Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins
Category
Protein Extraction & Purification, cell surface protein isolation
Kit's other name
none
Contents
• Quenching Solution • Lysis Buffer • Wash Buffer • Streptavidin Beads • PBS Tablet • TBS Tablet • Sulfo-NHS-SS-Biotin • DTT
Storage condition
4°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies
Samples tested
Adherent and suspension cells
Tissue
cell
Description
For cells, cell lines and tissues in culture till half confluency.