Information

Catalog number

K342-25

Full name

HDAC1 Immunoprecipitation (IP) & Activity Assay Kit

Size

25 assays

Price

834.00 €

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Details

Kit's description

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.

Highlights

• Detection method : Fluorescence (Ex/Em=380/500 nm) • Applications: : Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s) : Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme : Screening for activators or inhibitors of HDAC1

Kit's benefits

• Simple, fast and convenient. • Only kit on the market for both IP as well as measuring activity of HDAC1

Category

HDAC

Kit's other name

Histone Deacetylase 1 Immunoprecipitation (IP) & Activity Assay Kit

Contents

• HDAC Assay Buffer • Cell Lysis Buffer • HDAC Substrate • Developer • AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM) • Rabbit HDAC1 Antibody • Rabbit IgG (Control Antibody) • Protein-A/G Sepharose Beads (50% slurry in 20% ethanol/H2O) • Positive Control (Jurkat Cell Lysate) Lyophilized

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 moths

Species reactivity

see datasheet

Applications

Inquire

Samples tested

Human, Mouse or Rat • Cell Lysate and Nuclear Extract • Tissue Extract

Test

Biovision supplies other types of Assays as 1.