Information

Catalog number

K347-100

Full name

NADP/NADPH Quantitation Colorimetric Kit

Size

100 assays

Price

581.00 €

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Details

Kit's description

Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.

Highlights

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio

Kit's benefits

• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio • Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)

Category

NADP/NADPH

Kit's other name

none

Contents

• NADP/NADPH Extraction Buffer • NADP Cycling Buffer • NADP Cycling Enzyme Mix • NADPH Developer • Stop Solution • NADPH Standard (MW:833.36)

Storage condition

-70°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Inquire

Samples tested

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.