Information

Catalog number

K815-100

Full name

GFP Quantitation Kit

Size

100 assays

Price

399.00 €

Go to shop   

Details

Kit's description

Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea Victoria (Chalfie et al, 1994). GFP has also been recombinant modified and enhanced (called EGFP). GFP and EGFP have been widely used as a valuable reporter molecule for in vivo visualization of gene expression events in a variety of cell types and organisms. Since GFP requires no additional substrates or cofactors, GFP fluorescence can be easily detected under fluorescence microscope. However, most imaging studies of GFP are only qualitative. Since quantitative analysis of GFP expression level in cells or tissues are more informative and have wide applications, BioVision has developed the GFP Quantification Kit which quantifies GFP in 96 micro-plate format. Cells or tissues can be homogenized directly in the GFP Assay Buffer. The quantity of GFP is determined by comparing its fluorescence with that of GFP standard. The kit can detect a wide range of GFP concentration (0.01-10 µg/ml). A GFP quench solution is also provided for determining auto-fluorescence of cell or tissue extracts. Each kit provides sufficient reagents to perform up to 100 assays, Ex/Em = 488/507 nm.

Highlights

• Detection method: Fluorescence (Ex/Em 488/507 nm) • Species reactivity: Mammalian • Application: It detects a wide range of GFP concentration (0.01:10 ug/ml)

Kit's benefits

• Simple procedure; takes ~ 20 minutes • Fast and convenient

Category

GFP

Kit's other name

none

Contents

• GFP Assay Buffer • GFP Standard (1 µg/µl) • GFP Quench Solution

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Inquire

Samples tested

Cell and tissue cultures