Information
Catalog number
K1445-100
Full name
Plasmid DNA extraction and purification kit
Size
100 test
Price
425.00 €
Go to shopDetails
Storage Conditions
RT and 2-8°C as described in the datasheet
Tag Line
Plasmid DNA extraction and purification
Shipping Conditions
RT
Shelf life
12 months
Applications
Extraction and purification of plasmid DNA from bacteria culture.
Sample Type
bacteria culture
Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.