Information

Catalog number

P107

Full name

Exosome Isolation kit (for stem cell culture media)

Size

10

Price

600.00 €

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Details

Shipping/storage recommendations

room temperature

Maximum time for usage

12 months

Tissue

cell, stem, exosome

Description

For cells, cell lines and tissues in culture till half confluency.Differentiation and cell culture media are buffered growth medium solutions or culture medium solutions that can be supplied as a solid or liquid designed to support the growth of microorganisms or cells, or small plants. There are different types of media for growing different types of cells.

Gene

Exosomes are 50 nm small lipoprotein vesicles containing lots of microRNAs. The diagnostic value of exosomic miRNAs is due to their role in signal transduction and cell differentiation. Exoslomal total RNA can be analyzed by next gen sequencing to perform a diagnostic micro RNA profile.

Test

Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.