Information
Catalog number
STA-324
Full name
OxiSelect Oxidative DNA Damage Quantitation Kit (AP sites)
Size
50 assays
Price
780.00 €
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Shipping Temp
Room Temp
Storage Temp
Upon receipt, aliquot and store both the Reduced DNA and ARP-DNA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Category (Product Type)
Kit
URL link to SDS
UNSPSC
41116100
Product Line
Oxidative Stress/Damage
Short Description
DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect™ Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.
Product feature 1
Detect as few as 4-40 AP sites in 10^5 bp DNA
Product feature 2
Suitable for use with cells or tissues
Product feature 3
Oxidized and reduced DNA standards included for absolute quantitation