Information
Catalog number
AS08 322
Full name
Exo1, exoglucanase isoenzyme 1
Size
100 µl
Price
515.00 €
Go to shopDetails
Available ordering format
Lyophilized
Immunogen
synthetic peptide conjugated to KLH
Raised in
Rabbit
Clonality
Polyclonal
Clone
Polyclonal
Purification
Serum
How to reconstitute
For reconstitution add 100 µl of sterile water
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications
Western blot (WB), ELISA (ELISA)
Connected products
no related to other products
Recommended dilutions for use
1: 5 000 with standard ECL (WB) , 1: 10 000 (ELISA)
Molecular weight (expected | аpparent)
66 kDa
Verified reactivity
Hordeum vulgare
Possible reactivity
Oryza sativa, Zea mays
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
Supplementary information
to be added when available
References
to be added when available
Scientific context
Family 3 beta-d-glucan glucohydrolases are widely distributed in higher plants. The enzymes catalyse the hydrolytic removal of beta-d-glucosyl residues from non-reducing termini of a range of beta-d-glucans and beta-d-oligoglucosides. Their broad specificity can be rationalized from X-ray crystallographic data obtained from a barley beta-d-glucan glucohydrolase in complex with non-hydrolysable S-glycoside substrate analogues, and from molecular modelling of enzyme-substrate complexes. The glucosyl residue occupying binding subsite -1 is tightly locked into a fixed position through extensive hydrogen bonding with six amino acid residues near the bottom of an active site pocket. In contrast, the glucosyl residue at subsite +1 is located between two tryptophan residues at the entrance of the pocket, where it is less tightly constrained. The relative flexibility of binding at subsite +1, coupled with the projection of the remainder of bound substrate away from the enzyme-™s surface, means that the overall active site can accommodate a range of substrates with variable spatial dispositions of adjacent beta-d-glucosyl residues. The broad specificity for glycosidic linkage type would enable the enzyme to perform diverse functions during plant development.
Notes
None
Protein number
Refer to NCBI
TAIR number
Refer to NCBI