Information
Catalog number
AS08 370
Full name
ATPase, ATP synthase, whole enzyme
Size
100 µl
Price
550.00 €
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Available ordering format
Lyophilized
Immunogen
Purified native protein from spinach
Raised in
Rabbit
Clonality
Polyclonal
Clone
Polyclonal
Purification
Serum
How to reconstitute
For reconstitution add 50 µl of sterile water. To get the best possible dissolution please keep it at +4°C ON.
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications
Western blot (WB)
Connected products
AS08 304 | anti-ATP synthase subunit alpha antibodyAS03 030 | anti-ATP synthase subunit beta hen antibodyAS08 312 | anti-ATP synthase subunit gamma antibodyAS05 071 | anti-ATP synthase subunit c antibodyAS12 1853 | anti-ATP synthase subunit c antibodyPlant and algal protein extraction bufferSecondary antibodies
Recommended dilutions for use
1: 10 000 (WB)
Molecular weight (expected | аpparent)
38-58 kDa
Verified reactivity
subunits alpha, beta, gamma, epsilon of Ch. reinhardtii, S. oleracea, P.sativum, perennial grasses: Eremochloa ophiuriudes, Paspalum vaginatum
Possible reactivity
n. a.
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
Supplementary information
the prominent reaction is to a doublet around 55-58 kDa which is alpha and beta and weaker reaction to gamma subunit which is around 38 kDa
References
Liu et al. (2011). Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance. J Plant Physiol. Nov 7
Scientific context
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
Notes
None
Protein number
Refer to NCBI
TAIR number
Refer to NCBI
Tissue
whole
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.