Information

Catalog number

AS09 454

Full name

GDE / AGL, Glycogen debranching enzyme

Size

200 µg

Price

588.00 €

Go to shop   

Details

Available ordering format

Lyophilized

Immunogen

KLH-conjugated peptide derived from the sequence of human glycogen debranching enzyme P35573

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Affinity purified serum

How to reconstitute

For reconstitution add 200 µl of sterile water

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB), immunoprecipitation (IP)

Connected products

AS09 455 | anti-glycogen phosphorylaseSecondary antibodies

Recommended dilutions for use

1: 2000 (WB), 5 µg (IP)

Molecular weight (expected | аpparent)

175 kDa

Verified reactivity

human, mouse, rabbit,rat

Possible reactivity

dog

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

samples used to test this antibody were: muscle and liver homogenates, purified glycogen

References

Oldenburg et al. (2016). CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL). BMC Cancer. 2016 Sep 5;16:713. doi: 10.1186/s12885-016-2756-5.Pagliarani et al. (2014). Glycogen storage disease type III: A novel Agl knockout mouse model. Biochim Biophys Acta. 2014 Aug 1. pii: S0925-4439(14)00250-6. doi: 10.1016/j.bbadis.2014.07.029.

Scientific context

Synonymes:Glycogen debrancher, 4-alpha-glucanotransferase (EC=2.4.1.25), Oligo-1,4-1,4-glucantransferase, amylo-alpha-1,6-glucosidase, amylo-1,6-glucosidase (EC=3.2.1.33), Dextrin 6-alpha-D-glucosidase,amylo-1, 6-glucosidase, 4-alpha-glucanotransferase

Notes

This antibody can detect GDE in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GDE.Antibody will work as an excellent marker for glycogen. In tissues with no glycogen extression there will be no signal using this antibody.

Protein number

P35573

TAIR number

Refer to NCBI

Description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.