NAD-ME, Mitochondrial NAD-dependent malic enzyme


Catalog number

AS16 3932

Full name

NAD-ME, Mitochondrial NAD-dependent malic enzyme


50 µl


434.00 €

Go to shop   


Available ordering format



Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves.

Raised in







Serum + 0.01% sodium azide

How to reconstitute

For reconstitution add 50 µl of sterile water.

Storage condition

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB), immunolocalization (IL), immunoprecipiatation (IP)

Connected products

collection of antibodies to proteins involved in photosynthesiscollection of antibodies to mitochondrial proteinsPlant and algal protein extraction buffer Secondary antibodies

Recommended dilutions for use

1 : 1000 (WB), 1: 25 to 1: 100 (IL)

Molecular weight (expected | аpparent)

65 kDa

Verified reactivity

Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays

Possible reactivity

Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera

No reactivity

No confirmed exceptions from predicted reactivity are currently known.

Supplementary information

This antibody recognizes the large subunit (MEL) of the NAD-ME.Immunolocalization method is described in Long et al. (1994).


Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33.

Scientific context

NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.


Antibody can be used in immunolocalization using immunogold TEM.

Protein number

Refer to NCBI

TAIR number

Refer to NCBI




Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.