Information

Catalog number

ZU054

Full name

Streptavidin : Peroxidase Enzyme Conjugates from

Size

0.5 mg

Price

262.00 €

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Details

Availability

Please contact us to check the availability

Specificity

Binding to Biotin and Biotin conjugates

Species reactivity

not applicable

Product presentation

Conjugate in buffer solution containing Merthiolate** as a preservative

Product category

Enzyme Conjugates

Antibody's raised in

Streptomyces avidinii

Antibody's isotype

none

Antibody's clone

none

Product is supplied as

liquid concentrate

Recommended concentration for use

dilute 1:100 - 1:1000

Protein concentration

1 mg/ml

Positive control

none

Pretreatment

none

Tested applications

IHC, ELISA , WB

Recommended secondary reagent

Suitable substrates for peroxidase reaction in IHC are AEC (3-Amino-9-Ethyl-Carbazol) Art. No. AE002 and DAB (3,3'-Diaminobenzidin) Art. No. DA002 which result in red and brown colours respectively. Furthermore VECTOR Nova Red (red brown) Art. No. SK-4800, VECTOR VIP (violet) Art. No. SK-4600 and VECTOR SG (blue grey) Art. No. SK-4700 maybe used. In immuno blots DAB (Art. No. DA002), TMB (VECTOR Art. No. SK-4400) or chemiluminescence substrate DuoLUX (Art. No. SK-6604) are recommended.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

Go to PubMed

Tips

Gentaur products are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative thimerosal (Merthiolate) is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

Conjugate

Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH