Information

Catalog number

E-EL-R0102-96T

Full name

Rat BACE1(Beta-Site APP Cleaving Enzyme 1) EIA Kit

Size

96Test

Price

492.00 €

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Details

Detection Type

Colormetric

Target Species

Rat

Target Synonym

ASP2, HSPC104, Beta Secretase 1, Memapsin 2, membrane-associated aspartic protease 2, aspartyl protease 2

Test principle

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat BACE1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BACE1. You can calculate the concentration of Rat BACE1 in the samples by comparing the OD of the samples to the standard curve.

Sensitivity

0.1ng/mL

Detection Range

0.16~10ng/mL

Target Name

BACE1

UNIProt ID

P56819

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Rat BACE1(Beta-Site APP Cleaving Enzyme 1) EIA Kit

Type

Sandwich

Description

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

Properties

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus