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Mouse ECE1(Endothelin Converting Enzyme 1) EIA Kit

Information

Catalog number

E-EL-M0458-96T

Full name

Mouse ECE1(Endothelin Converting Enzyme 1) EIA Kit

Size

96Test

Price

492.00 €

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Details

Detection Type

Colormetric

Target Species

Mouse

Target Synonym

ECE1

Test principle

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECE1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ECE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ECE1. You can calculate the concentration of Mouse ECE1 in the samples by comparing the OD of the samples to the standard curve.

Sensitivity

9.38 pg/mL

Detection Range

15.625~1000pg/mL

Target Name

ECE1

UNIProt ID

Q4PZA2

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Mouse ECE1(Endothelin Converting Enzyme 1) EIA Kit

Type

Sandwich

Description

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

Properties

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus