Information
Catalog number
E-EL-0125-96T
Full name
A-CoA(Acetyl Coenzyme A) EIA Kit
Size
96Test
Price
433.00 €
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Detection Type
Colormetric
Target Species
Universal
Target Synonym
NA
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Universal A-CoA. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal A-CoA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal A-CoA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal A-CoA. You can calculate the concentration of Universal A-CoA in the samples by comparing the OD of the samples to the standard curve.
Sensitivity
0.19ng/mL
Detection Range
0.31~20ng/mL
Target Name
A-CoA
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
A-CoA(Acetyl Coenzyme A) EIA Kit
Type
Sandwich
Description
N terminal acetylation or CH3CO as epigenetic regulation of A-CoA( Coenzyme A) EIA Kit by NATs.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay