Information
Catalog number
E-CL-M0134-96T
Full name
Mouse BACE1 (Beta Site APP Cleaving Enzyme 1) CLIA Kit
Size
96Test
Price
541.00 €
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Detection Type
Colormetric
Target Species
Mouse
Target Synonym
ASP2, HSPC104, Beta Secretase 1, Memapsin 2, membrane-associated aspartic protease 2, aspartyl protease 2
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BACE1 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BACE1 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BACE1 . You can calculate the concentration of Mouse BACE1 in the samples by comparing the RLU value of the samples to the standard curve.
Sensitivity
18.75pg/mL
Detection Range
31.25~2000pg/mL
Target Name
BACE1
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Mouse BACE1 (Beta Site APP Cleaving Enzyme 1) CLIA Kit
Type
Sandwich
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus