Information
Catalog number
E-CL-H1386-96T
Full name
Human UBE2A (Ubiquitin Conjugating Enzyme E2A) CLIA Kit
Size
96Test
Price
541.00 €
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Detection Type
Colormetric
Target Species
Human
Target Synonym
NA
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBE2A . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE2A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE2A , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UBE2A . You can calculate the concentration of Human UBE2A in the samples by comparing the RLU value of the samples to the standard curve.
Sensitivity
37.5pg/mL
Detection Range
62.5~4000pg/mL
Target Name
UBE2A
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Human UBE2A (Ubiquitin Conjugating Enzyme E2A) CLIA Kit
Type
Sandwich
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.