Information
Catalog number
E-CL-H0190-96T
Full name
Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit
Size
96Test
Price
541.00 €
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Detection Type
Colormetric
Target Species
Human
Target Synonym
ASP2, HSPC104, Beta Secretase 1, Memapsin 2, membrane-associated aspartic protease 2, aspartyl protease 2
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BACE1 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BACE1 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BACE1 . You can calculate the concentration of Human BACE1 in the samples by comparing the RLU value of the samples to the standard curve.
Sensitivity
0.09ng/mL
Detection Range
0.16~10ng/mL
Target Name
BACE1
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit
Type
Sandwich
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.