Information
Catalog number
EKU09201
Full name
Mouse Ubiquitin Conjugating Enzyme E2I (UBE2I) ELISA Kit, 96 well plate
Size
96T
Price
872.00 €
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Verified reactivity
Mus musculus (Mouse)
Detection limits
0.312-20 ng/mL
Sensitivity limit
0,125 ng/mL
Samples to be analyzed
Tissue homogenates, cell lysates and other biological fluids
Assay duration
3 hours
Verified applications
ELISA
Assay class
Sandwich
ELISA detection
Colorimetric
Protein number
Please refer to Uniprot
Alternate protein number
Please refer to SwissProt
Gene number
Please refer to GenBank
Gene name
Ubiquitin Conjugating Enzyme E2I
Alternate gene name
P18; UBC9; UBCE9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-protein ligase I; SUMO-conjugating enzyme UBC9
Estimated production time
7-11 business days
Shipping requirements
Blue ice
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for
8 months
ELISA's specificity
This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2I (UBE2I). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2I (UBE2I) and analogues was observed.
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2I (UBE2I), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Conjugating Enzyme E2I (UBE2I) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Protocol
Please see ELISA's datasheet, otherwise contact us
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2I (UBE2I) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2I (UBE2I) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area
Enzyme & Kinase;
Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes
For research use only. Not for diagnostic procedures.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Latin name
Mus musculus