Information

Catalog number

WBFLA10-10

Full name

TruStrip WBV Flag-tag protein quantitation & Western Blot Validation Strips (10/pk)

Size

1 Pk

Price

218.00 €

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Details

Stock availability

Available

Category

Western Blot Validation Strips

Antibody type

N/A

Antibody host

N/A

Antibody conjugate

N/A

Technical datasheet

Contact Gentaur to request the datasheet or ask our specialists for more information.

Notes

The TruStrip WBV Flag-tag protein quantitation & Western Blot Validation Strips (10/pk) is manufactured for Research Use Only or for diagnostics purposes.

Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.

Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.

Conjugation

Flag