Information
Catalog number
20160-BT
Full name
Monoclonal Anti-Rabbit IgG (Light-chain) IgG-Biotin Conj (adsorbed, for immunoprecipitation/WB)
Size
0.5 ml
Price
253.00 €
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Stock availability
Available
Category
Secondary Antibodies
Antibody type
Monoclonal Antibody
Antibody host
Mouse
Antibody conjugate
Biotin
Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
Notes
The Monoclonal Anti-Rabbit IgG (Light-chain) IgG-Biotin Conj (adsorbed, for immunoprecipitation/WB) is manufactured for Research Use Only or for diagnostics purposes.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by adi. Rabbit antibodies are very stable and can be stored for several days at room temperature. adi adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Additional isotype
IgG
Latin name
Oryctolagus cuniculus