Information
Catalog number
AKR-106
Full name
Phosphoprotein Purification test kit
Size
5 preps
Price
511.50 €
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We send this reagant on
Room temperature
The temperatureerature for storage is
4ºC
Technical file
SDS or safty data sheet
Type of reagent
Cell Signaling and Protein Biology
Related techniques
Cell Signaling and Protein Biology, Protein Analysis Tools, Protein Phosphorylation, Phosphoprotein Purification test kit
Use of the reagent
The Phosphoprotein Purification test kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
Cellular use
Phosphorylated proteins are affinity purified from lysates with a single-step, purification matrix.
Protocol information
Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
Reagent properties
Entire procedure takes about 4 hours
Conjugation and form
host specific
Species compatible to
human cells and cell lines and other cells
Use of this test kit or product
Molecular biology
Antigen
the sequence of the protein
Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,10
Molecular weight
kiloDalton
This reagent is purified
0.98
Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Cellular biology labs supplies purification kits and ultra pure reagents.