Information
Catalog number
STA-327
Full name
OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit (CPD)
Size
96 assays
Price
693.50 €
Go to shopDetails
We send this reagant on
Room temperature
The temperatureerature for storage is
4ºC
Technical file
SDS or safty data sheet
Type of reagent
Oxidative Stress/Damage
Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, UV Induced DNA Damage test kits
Use of the reagent
Our OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
Cellular use
Measure CPD structures of DNA lesions induced by UV light
Protocol information
Detect structures by immunofluorescence staining under a fluorescence microscope
Species compatible to
Humans
Use of this test kit or product
Genetics lab
Antigen
a specific protein sequence
Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,67
Molecular weight
in kilo Danlto or kDa
This reagent is purified
0.97
Tissue
cellular
Description
Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.