Information
Catalog number
STA-321
Full name
OxiSelect™ DNA Double-Strand Break (DSB) Staining test kit
Size
100 assays
Price
693.50 €
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We send this reagant on
Blue Ice
The temperatureerature for storage is
-20ºC
SDS or safty data sheet
Type of reagent
Oxidative Stress/Damage
Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, DNA Double-Strand Break Assay
Use of the reagent
Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect™ DNA Double-Strand Break Staining test kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.
Cellular use
Detects phosphorylation of histone H2A.X
Protocol information
See immunofluorescence in about 3 hours
Reagent properties
DNA double-strand break inducer included
Species compatible to
Humans
Use of this test kit or product
Genetics lab
Antigen
a specific protein sequence
Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,50
Molecular weight
in kilo Danlto or kDa
This reagent is purified
0.97