Information

Catalog number

KTP20306-1-5

Full name

PurKine Biotin-Tag Protein Purification Kit (Streptavidin)

Size

5x1000ul

Price

482.00 €

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Details

Application

protein purification

Shipping conditions

The product PurKine Biotin-Tag Protein Purification Kit (Streptavidin) must be shipped on blue ice packages to retain the quality and activity of the reagents during the transportation.

Storage conditions

The product PurKine Biotin-Tag Protein Purification Kit (Streptavidin) can be kept between four and eight degrees Celsius for 12 months upon receipt.

Tips

This kit is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Test

Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Abbkine supplies purification kits and ultra pure reagents.