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OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit (6-4PP)

Information

Catalog number

STA-329

Full name

OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit (6-4PP)

Size

96 assays

Price

693.50 €

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Details

We send this reagant on

Room temperature

The temperatureerature for storage is

4ºC

Technical file

kit.pdf

SDS or safty data sheet

(STA-329) GHS.pdf

Type of reagent

Oxidative Stress/Damage

Related techniques

Oxidative Stress/ Damage, DNA / RNA Damage and Repair, UV Induced DNA Damage test kits

Use of the reagent

Our OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

Cellular use

Measure 6-4PP structures of DNA lesions induced by UV light

Protocol information

Detect structures by immunofluorescence staining under a fluorescence microscope

Species compatible to

Humans

Use of this test kit or product

Genetics lab

Antigen

a specific protein sequence

Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,71

Molecular weight

in kilo Danlto or kDa

This reagent is purified

0.97

Tissue

cellular

Description

Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.