2, Photosynthesis Tool Kit - quantitation
50 µl of respective antibody. 100 µl / tube of each protein standard. 50 µl of secondary antibody
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Available ordering format
KLH-conjugated syntetic peptides for respective antibodies
Rabbit. Secondary antibody: Goat.
How to reconstitute
See included datasheet or contact our support service
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
western blot (WB)
Plant and algal protein extraction bufferSecondary antibodies
Recommended dilutions for use
Molecular weight (expected | аpparent)
higher plants, algae, cyanobacteria
to be determined
to be determined
Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies. References: Brown et al. (2007). Resource dynamics during infection of Micromonas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202. Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951. Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)
This kit allows quantitation of major photosynthetic proteins: Rubisco, PsaC (PSI) and PsbA (PSII) as well as determination of PSI/PSII ratios using the western blot technique. The kit contains following global antibodies, protein standards and secondary anribody: Rabbit anti-RbcL (Form I and Form II). Product number: AS03 037 Rabbit anti-PsaC. Product number: AS10 939Rabbit anti-PsbA Product number: AS05 084Rubisco protein standard. Product number: AS01 017S PsaC protein standard. Product number: AS04 042S PsbA protein standard. Product number: AS01 016S Goat anti-Rabbit IgG (H&L), HRP conj. (50 µl), Product number: AS09 602
Product information - Primary antibodies:Product nuumber:Product name:Reconstitution: Recommended dilution:AS03 037Rabbit Anti-RbcL Global antibodyFor reconstitution see lable on respective tube.1:5000-10 000 with standard ECLAS10 939Rabbit Anti-PsaC Global antibodyFor reconstitution see lable on respective tube.1:1000 with standard ECLAS05 084Rabbit Anti-PsbAGlobal antibodyFor reconstitution see lable on respective tube.1:10 000 with standard ECL* All primary antibodies in this kit are raised in rabbits.Product information - Protein standards:Product number:Product name:Concentration:Size:Western Blot - Positive Control:AS01 016SPsbA *0.25 pmol/ Î¼l41.5 kDa#To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.AS01 017SRbcL *0.15 pmol/ Î¼l52.7 kDaTo generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range.A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.AS04 042SPsaC *0.15 pmol/Î¼l11.5 kDa#To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 Âµl). For most applications a sample load of 0.2 Âµg of chlorophyll will give a PsaC signal in this range.A 2 ul load is optimal for most chemiluminescent detection systems as a positive control.*These proteins are larger than a respective native protein due to the addition of His-tag* For reconstitution of standards see lable on respective tube.Product information - Secondary antibody:AS09 602- Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 Âµl (2x25 Âµl)Educational information about Quantitative western blot can be found here: detailed method description, video tutorial
O03042 , P05698 , P0C510 , P00877 , P62090 , P69416 , P0C360 , Q00914 , Q31QV2 , P83755 , P0C434 , Q14FH6 , Q6YXN7 , P07753 , P14660