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M’Fadyean Stain (Polychrome Methylene Blue)

Information

Catalog number

S047-40X125ML

Full name

M’Fadyean Stain (Polychrome Methylene Blue)

Size

40X125ML

Price

150.00 €

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Details

Description

M’Fadyean Stain (Polychrome Methylene Blue) is a readymade stain reported to be used for capsule staining of Bacillus anthracis

Application

For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.

Classification

Capsule Stains

Composition

Methylene blue 0.300 gm 95% ethyl alcohol 30.000 ml Potassium hydroxide 0.010 gm Distilled water 100.000 ml

Appearance

Deep violet coloured solution

Clarity

Clear without any particles.

Microscopic Examination

Capsule staining is carried out using M'Fadyean stain and staining characteristic is observed under microscope using oil immersion lens.

Results

Bacillus anthracis ATCC 14578 : Pink capsule seen surrounding the deep bluish bacilli. Bacillus cereus ATCC 10876 : Deep bluish bacilli seen.

Storage

Store below 30°C in tightly closed container and away from bright light.

Shelf Life

Use before expiry date on label.

Properties

Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.

Conjugation

Blue Substrate