Information

Catalog number

5522

Full name

Amplite Colorimetric Biotin Quantitation Kit

Size

200 Tests

Price

345.00 €

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Details

Description

Amplite Colorimetric Biotin Quantitation Kit is used for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

Shipping

Room Temperature

Storage

Freezer

Shelflife

6 months

Kit Components

Component A: avidin ; Component B: HABA Assay Buffer; Component C: D-Biotin

Ex (nm)

500 nm

Em (nm)

NA

Personal Protection

Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.

Uses

Laboratory chemicals *For Research Use Only*

Ordering

To order Amplite Colorimetric Biotin Quantitation Kit, please use the Cat. Nr.5522 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information

Tips

Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Colorimetric Biotin Quantitation Kit

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Conjugation

Biotinylated