Menu

Live or Dead Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*

Information

Catalog number

22599

Full name

Live or Dead Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*

Size

200 Tests

Price

345.00 €

Go to shop   

Details

Description

Live or Dead Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry* is a kit that is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation.

Shipping

Room Temperature

Storage

Freezer

Shelflife

6 months

Chemical components

Component A: Stain It™ Reactive Dye ; Component B: DMSO

Excitation Wavelength

523 nm

Emission Wavelength

617 nm

Personal precautions

Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.

Uses

Laboratory chemicals *For Research Use Only*

Ordering

To order Live or Dead Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*, please use the Cat. Nr.22599 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information

Tips

Our specialists recommend you to follow carefully the pre-registered instructions for Live or Dead Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*

Tissue

cell

Additional description

For cells, cell lines and tissues in culture till half confluency.FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.

Test

Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

Conjugation

red dye