Information
Catalog number
NE001-Bio
Full name
Rabbit anti Saccharomyces cerevisiae S-Acetyl Coenzyme A synthetase, conjugated with Biotin, Primary Antibodies
Size
1ml
Price
487.00 €
Go to shopDetails
Host/Source
Rabbit
Clone Name
Polyclonal
Product Category
Enzymes, Microbiology
Species Reactivity
Saccharomyces cerevisiae
Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
Immunogen
S-Acetyl coenzyme A synthetase isolated and purified from bakerÍs yeast. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
Purification Method
NA
Shipping Conditions
NA
Storage conditions
Rabbit anti Saccharomyces cerevisiae S-Acetyl Coenzyme A synthetase, conjugated with Biotin, Primary Antibodies philised conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days.
Datasheet link
NA
Description
N terminal acetylation or CH3CO as epigenetic regulation of Rabbit anti Saccharomyces cerevisiae S- Coenzyme A synthetase, conjugated with Primary Antibodies by NATs.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
About
Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus