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Protein G Agarose antibody Antibody Purification Reagent

Information

Catalog number

X1196

Full name

Protein G Agarose antibody Antibody Purification Reagent

Size

5mL

Price

259.00 €

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Details

Category

Other Reagents

Long description

This outstanding recombinant Protein G has the albumin binding domain deleted providing very clean immunoglobin purifications. It is coupled to highly cross-linked agarose at 2 mg recombinant Protein G per ml beads using cyanogen bromide activation. The combination provides very superior performance binding 20 mg of human IgG per ml beads under static conditions. Binding under flow will be less depending on the flow rate for all Protein A and Protein G products. High flow rates are possible with this highly cross-linked agarose product under gravity flow, but it is not designed to pump under high pressure.

Antibody come from

n/a

Other description

Provided as a 50% aqueous suspension of Protein G in 20% ethanol solution.

Clone

not specified

Antigen-antibody binding interaction

Protein G Agarose antibody Antibody Purification Reagent

Antibody is raised in

see techfile

Antibody's reacts with

IgG

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

IgG Purification

Antibody's suited for

LIGAND DENSITY: approximately 2 mg Protein G /mL of drained gel_x000B_DYNAMIC BINDING CAPACITY: approximately 18 mg human IgG/ml of drained gel (calculated as the amount of human IgG adsorbed to the gel before flow exceeded 1% of the absorbance of the ingoing solution at 30 cm/hr and a 0.92 mg/ml sample concentration.)_x000B_BEAD STRUCTURE: 4% highly crossed linked agarose_x000B_BEAD SIZE RANGE: 45-185 µm_x000B_MAXIMUM LINEAR FLOW RATE:>1300 cm/hr at 0.05 MPa (0.5 bar) in an XK 16/20 column, bed height 5 cm_x000B_MAXIMUM OPERATING BACK PRESSURE: 0.1 MPa (1 bar, 14 psi)_x000B_pH WORKING RANGE: 2-9 (Note: Protein G may hydrolyze at low pH)_x000B_pH STABILITY: long term storage and cleaning in place- pH of 3-9_x000B_CHEMICAL STABILITY: IgG binding capacity and recovery maintained after store for 7 days at_x000B_ 1) 37¼C in 1M acetic acid (pH 2.0), 2mM sodium phosphate, 1% sDS (pH 7.0), 6 M guanidine- HCL, 70% ethanol or_x000B_ 2) 2 hrs at room temperature in 0.1 M HCL, pH 1.0, 8 M urea, pH 10.5, 01 M Glycine-NaOH, ph 11_x000B_PHYSICAL STABILITY: Negligible volume variation due to changes in pH or ionic strength_x000B_SANITIZATION: Sanitize column using 70% ethanol_x000B__x000B_

Storage

4ºC

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Properties

The purest agarose was used in the production of Protein G Agarose antibody Antibody Purification Reagent by nordc.If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Conjugation

Agarose

Test

Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.

French translation

anticorps