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HDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme

Information

Catalog number

X1737E

Full name

HDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme

Size

20 μg

Price

426.00 €

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Details

Category

Proteins & Peptides

Long description

Acetylation of the tail of histone is known to cause chromatin to adopt a more ÔopenÕ 3D conformation, allowing trans factors grerater access to DNA. Histone acetyltransferases (HATs), complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, HATs acetylate non-histone proteins, implicating them in a wide variety of regulartory roles for these enzymes. By comparison, histone deacetylation promotes a more ÔclosedÕ chromatin conformation and in general leads to repression of gene activity. Mammalian histone deacetylases are divided into three classes on the basis of their similarity to various the yeast deacetylases. Class I (HDACs 1, 2, 3 and 8) related to the yeast Rpd3-like proteins, class II (HDACs 4, 5, 6, 7, 9 and 10) related to yeast Hda1-like proteins and class III related to the yeast protein Sir2. Inhibitors of HDAC have enormous potential as cancer therapeutic agents.

Antibody come from

Hybridoma produced by the fusion of splenocytes from Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface and mouse Sp2/0 myeloma cells.

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Clone

not specified

Antigen-antibody binding interaction

HDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme

Antibody is raised in

Sf9 cells

Antibody's reacts with

Human

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antibody's specificity

No Data Available

Application

Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Antibody's suited for

Antibody suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on frozen sections and flow cytometry (1:200 dilution). Optimal concentration should be evaluated by serial dilutions.

Storage

-20ºC

Relevant references

1. Hallmann, R., et al. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9._x000B__x000B_2. Hammel, M., et al. (2001). Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74._x000B_

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.

Conjugation

Flag,histidine